Course Date:  July 25-27, 2017

Course Description:

Individuals attending this course gain a fundamental knowledge of the latest, most advanced cloning strategies vital to mammalian cell line development for protein production. Emphasis is on the choice of the expression vector, the host cell genetics and physiology, and screening strategies to assure the highest production yields using Chinese hamster ovary (CHO) cells as the expression model. Practical hands-on laboratory sessions include DNA cloning, cell transfection, selection/screening for best producer, and the expression/analysis of the protein of interest. This course is useful to professionals with new assignments that include mammalian cell line development and to scientists and engineers with experience in other aspects of molecular biology planning or with limited experience in molecular biology/genetic expression.